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Effect of Rosmarinic Acid on the Focal Adhesions of MC3T3-E1 Preosteoblasts on Titanium Surface
J Dent Hyg Sci 2024;24:181-9
Published online September 30, 2024;  https://doi.org/10.17135/jdhs.2024.24.3.181
© 2024 Korean Society of Dental Hygiene Science.

Moon-Jin Jeong1 , Myoung-Hwa Lee1 , Do-Seon Lim2 , and Soon-Jeong Jeong3,4,†

1Department of Oral Histology and Developmental Biology, School of Dentistry, Chosun University, Gwangju 61452, 2Department of Dental Hygiene, Graduate School of Public Health Science, Eulji University, Seongnam 13135, 3Department of Dental Hygiene, College of Health Science, Youngsan University, Yangsan 50510, 4Institute of Basic Science for Well-Aging, Youngsan University, Yangsan 50510, Korea
Correspondence to: Soon-Jeong Jeong, https://orcid.org/0000-0002-8959-4663
Department of Dental Hygiene, College of Health Science, Youngsan University, 288 Junam-ro, Yangsan 50510, Korea
Tel: +82-55-380-9453, Fax: +82-55-380-9305, E-mail: jeongsj@ysu.ac.kr
Received September 2, 2024; Revised September 7, 2024; Accepted September 11, 2024.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Focal adhesions (FAs) is the most important process in the first step of osseointegration between preosteoblasts and titanium (Ti). FAs improvement and pre-osteoblasts cell proliferation leads to successful Ti-based dental implants. This study aimed to confirm the applicability of rosmarinic acid (RA) as a functional substance for improving FAs and cell proliferation of MC3T3-E1 preosteoblasts on Ti surfaces during the first stage of osseointegration for successful Ti-based dental implants.
Methods: We used MC3T3-E1 preosteoblasts on Ti discs incubated in a medium supplemented with or without 14 μg/ml to decipher the effects of RA on FAs and cell proliferation. FAs and proliferation of MC3T3-E1 cells on Ti discs were assessed via MTT assay. Actin-labeled cells and paxillin contacts were observed and imaged by fluorescent microscopy, and the associated signaling pathways were revealed through western blot analysis.
Results: In RA-treated MC3T3-E1 cells on Ti discs, FAs between MC3T3-E1 preosteoblasts and Ti surfaces and the expression of focal adhesion kinase (FAK), phosphorylated FAK and paxillin proteins and filamentous-actin formation increased. RA increased the proliferation of MC3T3-E1 preosteoblasts on the Ti surface as well as the expression of Grab2, Ras, pERK1/2, and ERK1/2. In addition, the expression of secretory leukocyte protease inhibitor and thymosin b4, known as nanomolecules that enhance the interaction between implanted Ti materials and preosteoblasts in the RA-treated MC3T3-E1 preosteoblasts, increased. RA not only increased the FAs of MC3T3-E1 preosteoblasts on the Ti surface through the FAK/Paxillin signaling pathway, but also increased cell proliferation and mitosis through the FAK/Grab2/Ras/ERK1/2 signaling pathway.
Conclusion: RA can be applied as an effective functional substrate to improve the FAs and proliferation of MC3T3-E1 preosteoblats on Ti surfaces, which are essential in the first step of osseointegration between implanted Ti and bone tissue for the clinical success of Ti based dental implants.
Keywords : Focal adhesions, MC3T3-E1 preosteoblast,Osseointegration, Rosmarinic acid, Titanium


September 2024, 24 (3)
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