Fig. 2. Effect of RA on focal adhesions in MC3T3-E1 preosteoblasts on Ti surface. After incubation of MC3T3-E1 cells on Ti discs for 12 hours in serum-free medium, the cells were incubated with or without 14 µg/ml RA, total proteins were extracted and analyzed via western blot using the indicated antibodies and β-actin was used as an internal control. (A) pFAK, FAK and paxillin expression. (B, C) Quantitative analysis of western blots. RA: rosmarinic acid, Ti: titanium, FAK: focal adhesion kinase, pFAK: phosphorylated FAK. *p<0.05, compared to control.