Journal of Dental Hygiene Science : eISSN 2233-7679

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Fig. 3. Effects of nicotine and rapamycin treatment on protein expression of autophagy markers and senescence-associated b-galactosidase (SA-b-gal) staining in HGF-1. Cells were treated with nicotine (1 mM) and rapamycin (10 nM) for 48 hours. (A) Protein expression of autophagy markers (LC3-Ⅱ, p62, and Beclin-1) in HGF-1 was determined by western blot analysis. Signals of each band were normalized to the respective b-actin. (B) Magnification ×100 and quantification of SA-b-Gal+ cells cultures. Values are mean±standard deviation of three experiments. *Statistically significant difference compared with control group (p<0.05). #Statistically significant difference compared with nicotine treatment group (p<0.05).
J Dent Hyg Sci 2022;22:164-70
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