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The Effects of Biofilm Care on Subgingival Bacterial Motility and Halitosis
J Dent Hyg Sci 2019;19:162-9
Published online September 30, 2019;
© 2019 Korean Society of Dental Hygiene Science.

Yu-Rin Kim

Department of Dental Hygiene, Silla University, Busan 46958, Korea
Correspondence to: Yu-Rin Kim,
Department of Dental Hygiene, Silla University, 140 Baegyang-daero 700beon-gil, Sasang-gu, Busan 46958, Kore
Tel: +82-51-999-5707, Fax: +82-51-999-5745, E-mail:
Received July 15, 2019; Revised August 7, 2019; Accepted August 20, 2019.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background: Oral diseases are caused by various systemic and local factors, the most closely related being the biofilm. However, the challenges involved in removing an established biofilm necessitate professional care for its removal. This study aimed to evaluate and compare the effects of professional self and professional biofilm care in healthy patients to prevent the development of periodontal diseases.
Methods: Thirty-seven patients who visited the dental clinic between September 2018 and February 2019 were included in this study. Self-biofilm care was performed by routine tooth brushing and professional biofilm care was provided using the toothpick method (TPM) or the oral prophylaxis (OP) method using a rubber cup. Subgingival bacterial motility and halitosis (levels of hydrogen sulfide, H2S; methyl mercaptan, CH3SH; and di-methyl sulfide, (CH3)2S) were measured before, immediately after, and 5 hours after the preventive treatment in the three groups. Repeated measures analysis of variance test was performed to determine significant differences among the groups.
Results:TPM was effective immediately after the prevention treatment, whereas OP was more effective after 5 hours (proximal surfaces, F=16.353, p<0.001; smooth surfaces, F=66.575, p<0.001). The three components responsible for halitosis were effectively reduced by professional biofilm care immediately after the preventive treatment; however, self-biofilm care was more effective after 5 hours (H2S, F=3.564, p=0.011; CH3SH, F=6.657, p<0.001; (CH3)2S, F=21.135, p<0.001).
Conclusion: To prevent oral diseases, it is critical to monitor the biofilm. The dental hygienist should check the oral hygiene status and the ability of the patient to administer oral care. Professional biofilm care should be provided by assessing and treating each surface of the tooth. We hope to strengthen our professional in biofilm care through continuous clinical research.
Keywords : Bacteria, Dental care, Halitosis, Oral health

September 2019, 19 (3)
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