Journal of Dental Hygiene Science : eISSN 2233-7679

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Fig. 3.

Total protein expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) by the light-emitting diodes curing unit (LCU) irradiation in MDPC-23 cells and lipopolysaccharide (LPS) stimulated MDPC-23 cells. The cells were irradiated with LCU for 20 seconds and LPS (100 ng/ml) was treated. (A) Total protein was extracted from the MDPC-23 cells and was subjected to western blot analysis using indicated antibodies. Actin was used as internal control for the western blot assays. The values of IL-1β (B) and TNF-α (C) in the culture supernatants were measured by commercial enzyme-linked immunosorbent assay kits. Value are represented the mean±standard deviation of results from three independent experiments. *p<0.05 and **p<0.01 compared with the control group. #p<0.05 and ##p<0.01 compared with the LPS groups.

J Dent Hyg Sci 2019;19:39-47
© 2019 J Dent Hyg Sci